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Genome modifications in microalgae are becoming a widespread and mandatory tool for research in both fundamental and applied biology. Among genome editing methods in these photosynthetic organisms, CRISPR/Cas9 offers a specific, powerful and efficient tool for genome engineering by inducing mutations in targeted regions of the genome. Here we described a protocol that allows the generation of knockout mutants by CRISPR/Cas9 in the diatom Phaeodactylum tricornutum using biolistic transformation.

Allorent G.*, Guglielmino E., Giustini C. and Courtois F. (2018) Generation of mutants of nuclear-encoded plastid proteins using CRISPR/Cas9 in the diatom Phaeodactylum tricornutum. Methods in Molecular Biology. 1829:367-378. doi: 10.1007/978-1-4939-8654-5_24.